DISCOVERY OF NOVEL EPIGENETIC BIOMARKERS IN ORAL MALIGNANT LESIONS BY EPIGENOMICS AND TRANSCRIPTOMICS APPROACHES

Objectives: DNA methylation, which is the most frequently observed epigenetic change, very important because it seen in early stages of cancer. Abnormal methylation promoter regions and silencing tumor suppressor genes play a key role development oral squamous cell carcinoma (OSCC). In our study*, was aimed to identify validate new biomarker candidates via epigenomics/transcriptomics approaches, understand molecular mechanisms OSCC discover biomarkers for diagnosis. Materials Methods: After DNA/RNA isolation tumor/matched-normal tissue samples from 6 patients, BC-DNA/cDNA synthesis performed, respectively. The expression profiles were analyzed by R(v3.5.1) environment methods using IlluminaHumanMethylation450chips IlluminaiScan, A candidate gene showing methylation-dependent loss after bioinformatic analysis validated QRT-PCR AND QMSP tissues body fluids 20 premalignant lesions (OPML) Results: To candidate, we selected show either hypermethylation lower patterns. This (unpublished data), belong subfamily protein-tyrosine kinase, found be methylated 65% tumors, 20% matched-normal OSCC. rates tumor, saliva OPML patients 55%, 40% 10%, decreased levels 55% 35% Conclusions: Our could detection OSCC.